Plant-tc Monthly Archive - July 2000

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Re: Intergeneric hybridization in orchid,



Instead of a Cesium source, could one use a common microwave oven to "denature"
the pollen?
Henry Kuska, retired
kuska@neo.rr.com
http://home.neo.lrun.com./kuska/
----- Original Message -----
From: "Lester W. Poole" <lwp@LAJOYA.COM>
To: <plant-tc@tc.umn.edu>
Sent: Friday, July 14, 2000 10:53 AM
Subject: Re: Intergeneric hybridization in orchid,


> J Devi...
>
> Do an abstract search of the American Orchid Societies orchid research
> publication 'Lindleyana'.  I don't recall any research done with orchids on
> invitro fertilization.  Maryland Light in Ottawa had done work with
> germinating pollen.  You may need to first trigger the ovary to develop
> receptive ova by inducing (excuse the term), a 'false pregnancy'.
>
> You can do this by having the pollen irradiated for about an hour, I used a
> Cesium chamber with my tests.. this should 'denature' (bad term) the pollen
> to prevent fertilization, but leave the triggering chemical structures in
> tack.  The process can result in developing pods without any fertilization
> occuring.  You should then be able to extract the placetal tissue from the
> developed ova and develop your processes with germinated pollen invitro. You
> may be able to also do the same with BA applied to the stigmatic surface.  I
> had been told a hybridizers paste has been used in the past quite
> successfully to induce ovary development using a 40 ppm gell of IAA (?).
>
> Question is  whether this will allow the natural process of fertilization to
> occur.  The structure of the orchid ovary is basically a six sided structure
> and the whole mechanism is nothing more than a long tube that is sealed at
> the floral end with a stigmatic jelly.  As the pollen germinates in the
> stigmatice jelly, the pollen tubes grow down this tube to the ova.  In the
> ovary this tube structure may help guide the pollen to the ovary ... question
> is, what guides the pollen tube to contact with the ova? Perhaps an
> electrical charge of some sort ... don't know.  Another question is how the
> lights in the lab environement will affect all this?
>
> If the problem you are running into is one of rapid pod rejection, then you
> may wish to try the BA or even IAA in a paste to see if that will allow the
> pod to remain on until you can sow.  Pod rejection a problem we don't have
> much information on ... whether the stigma has a mechanism to sort out the
> 'wrong' pollen chemicals or perhaps a lack or proper signal chemical induces
> the pod to abort.  I did work in the late seventies with 'Mentor Pollen
> Effects' on orchids that indicated  interesting potential  in this area.  I
> have an extensive background in commercial orchid hybridization and concepts,
> please feel free to contact me.
>

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