Re: contamination

[jumana serhan <jumana_fuad@YAHOO.COM>]

  i had the same  trouble ,we tried to increase the
sterilization to 20 min, to clean the jars very well,
and to have a very restricted culturing techniqes ....
  but we still have the trouble

--- Brent McCown <bhmccown@FACSTAFF.WISC.EDU> wrote:
> Probably insects.
>
> Brent
>
>
> At 08:32 AM 12/5/00 -0600, you wrote:
> >Dear Member,
> >We are facing some problems in contamination in tissue culture medium.
> >We are using magenta caps for the tissue culture bottles. The
> >contamination of bacteria and some times fungus comes after autoclaving
> >and storing the media at room temperature. We autoclave the bottles at
> >15 psi for 20 min. could anybody suggest from where the contamination
> >comes and at what precautionary measures could be taken to prevent the
> >same.
> >Thanks in advance.
> >Rishi

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