Re: Antibiotic concentration

Subject: Re: Antibiotic concentration
From: Brian Weir <B.Weir@PI.CSIRO.AU>
Date: Fri, 2 Feb 2001 08:59:15 +1100
Reply-To: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>
Sender: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>

Geraldine,
The concentration of any selection agent will not only vary greatly between
species, but can vary among cultivars within a species as well.  The
concentration can also depend on culture conditions, partiularly the
gelling agent used, and the timing of application. You can establish a
'kill curve' using non-transformed callus and shoots to determine an
appropriate level to distinguish between transgenic and non-transgenic plants.
Brian

At 15:36 1/02/01 +0900, you wrote:
>Dear All,
>
>I would like to know the concentration of hygromycin and phosphinothricin
>that is usually necessary to select transformed plants. I will transform
>Sunflower as well as Dandelion. Thanks in advance.
>
>Geraldine Blanc
>
>
Brian Weir
CSIRO Plant Industry

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