Re: Antibiotic concentration

[Geraldine BLANC <blanc@KSC.KUMHO.CO.KR>]

Dear Brian,

Thanks for your answer. I should have been more precise in my previous message.
I would like to know the range of concentrations that researchers usually
use in order to have an idea to start. Could you help me ? I use Agar for
gelling agent and I let the tissues 5 days on the selection medium.

Geraldine

At 08:59 AM 2/2/01 +1100, you wrote:
>Geraldine,
>The concentration of any selection agent will not only vary greatly between
>species, but can vary among cultivars within a species as well.  The
>concentration can also depend on culture conditions, partiularly the
>gelling agent used, and the timing of application. You can establish a
>'kill curve' using non-transformed callus and shoots to determine an
>appropriate level to distinguish between transgenic and non-transgenic plants.
>Brian
>
>At 15:36 1/02/01 +0900, you wrote:
> >Dear All,
> >
> >I would like to know the concentration of hygromycin and phosphinothricin
> >that is usually necessary to select transformed plants. I will transform
> >Sunflower as well as Dandelion. Thanks in advance.
> >
> >Geraldine Blanc
> >
> >
>Brian Weir
>CSIRO Plant Industry

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