Re: rooting of woody material

[Chuck Maynard <cmaynard@MAILBOX.SYR.EDU>]

Nceba,

I'm not sure why you would want to root in vitro.  Many commercial labs
are going the other way. After multiplying the material in vitro, they
remove it and root in sand or perlite under fog or mist.

One immediate problem is going to be contamination. Any surface
sterilization procedure I am familiar with causes considerable damage to
the surface tissues, often penetrating up the ends of cut stems a
considerable distance. This would be the very tissues you would be
depending on to form new roots.

You might try skipping the surface sterilization and load up the media
with antibiotics and systemic fungicides and/or eliminate the sucrose.

Agar, gelan gum, and the rest of the gelling agents do a good job of
holding up the plants, but few woody plants are aquatic enough to
actually LIKE rooting in what is basically stiff water.  I would suggest
substituting vermiculite or perlite for a gel media.

Best of luck,

Chuck Maynard
--
Dr. Charles Maynard
Forest Geneticist, Faculty of Forestry
SUNY College of Environmental Science
and Forestry
(315)470-6560
Fax: (315)470-6956
e-mail: cmaynard@mailbox.syr.edu
http://www.esf.edu/course/cmaynard/Maynard.html


Nceba wrote:

> Hello to you all
> I would love to know if the following is possible, I wanna induce
> rooting to a woody plant material so is it possible if I can collect
> the material from field and then try and root them in vitro, in spite
> the contamination problem, is it is possible to root a material
> straight from the field in vitro by passing all the other steps like
> establishment, multiplication, subculture.
> I would appreciate the feedback

---

[Subject Prev][Subject Next][Thread Prev][Thread Next]

---

Plant-tc Listserv Homepage | 
Subject Index | 
Thread Index