Re: Phaseolus

[Wayne Parrott <wparrott@ARCHES.UGA.EDU>]

At 04:01 PM 2/5/01 -0600, you wrote:
>Has anyone ever developed embryogenesis for Phaseolus vulgaris?
>-----Original Message-----
>From: Kevin Worden [mailto:WORDENK@STATE.MI.US]
>Sent: Monday, February 05, 2001 3:39 PM
>To: plant-tc@tc.umn.edu
>Subject: Phaseolus
>
>Greetings all,
>
>Does anyone know of a good protocol for Phaseolus vulgaris  embryogenesis ?
>
>Thank you.

There are tantalizing hints in the literature that it is possible, though a
successful protocol has never been developed to my knowledge.  It is
certainly possible in related species.  I would love to know if anyone else
has additional information:

Allavena,A. and L.Rossetti. 1983. Efforts in somatic embryogenesis of
Phaseolus vulgaris L. Acta Hort. 131:239-247.

Martins,I.S. and M.R.Sondahl. 1984. Early stages of somatic embryo
differentiation from callus cells of bean (Phaseolus vulgaris L.) grown in
liquid medium. J. Plant Physiol. 117:97-103.
Notes: Got early stages of embryogenesis, but would not proceed beyond the
globular stage.

Saunders,J.A., G.L.Hosfield, and A.Levi. 1987. Morphogenetic effects of
2,4-dichlorophenoxacetic acid on pinto bean (Phaseolus vulgaris L.) leaf
explants in vitro. Plant Cell Rep. 6:46-49.
Notes: Got what looks like embryogenic callus from immature leaflets on 20
mg/L 2,4-D, which could be maintained as "self-replicating" globular
embryos in liquid with 30 mg/L.  Never proceeded past globular stage, which
is not unexpected given the high 2,4-D level

Wayne Parrott
Dept. Crop & Soil Sciences
University of Georgia
Athens, GA 30602-7272
(706) 542-0928/fax 0914
http://www.cropsoil.uga.edu/~parrottlab

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