Re: Xanthi RNA prep

Subject: Re: Xanthi RNA prep
From: David A Wright <wrightd@IASTATE.EDU>
Date: Mon, 5 Feb 2001 18:31:48 -0700
Reply-To: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>
Sender: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>

I have been using an RNA kit from Gentra Systems Inc
(http://www.gentra.com/).  It is called the Purescript RNA isolation kit,
the Catalog number is R-5000A.  There are two solutions and there is enough
to make a lot of RNA preps.  The RNA seem to be of good quality as I have
been able to RT-PCR poly A transcripts of up to 8.4Kb from Arabidopsis.  Of
all the RNA kits and protocols I have tried, this has been the best by far.

David Wright

>Hello all!
>I am searching for a new way to prep RNA from transgenic tobacco tissue, for
>the TRizol I have been using isn't doing a great job.  The more simplified
>the better, seeing that i am screening through hundreds of plants!
>
>Thanks a lot,
>sara spencer

David A. Wright, Ph.D.

Iowa State University
2278 Molec. Bio. Bldg.
Ames, Iowa  50011

Phone: (515) 294-0335
Fax: (515) 294-6755

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