Re: Phaseolus

[giorgis kaparakis <Giorgis.Kaparakis@GMX.NET>]

 hi!
I aware of this article  about the induction of somatic embryogenesis of 
Phaseolus vulgaris.

MALIK, K.A. and SAXENA, P.K. (1992) Regeneration in Phaseolus vulgaris 
L.: High frequency induction of direct shoot formation in intact 
seedlings by N6-benzylaminopurine and thidiazuron.  Planta 186, 384-389.

from using the same protocol (culture of seeds in solid media containing 
BAP or TDZ) somatic embryogenesis have been induced in peanut

Murthy, B.N.S., Murch, S.J. and Saxena, P.K. (1995) Thidiazuron-induced 
somatic embryogenesis in intact seedlings of peanut (Arachis hypogea): 
Endogenous growth regulator levels and significance of cotyledons.  
Physiologia Plantarum 94, 268-276.

Victor, J.M.R., Murch, S.J., KrishnaRaj, S. and Saxena, P.K. (1999) 
Somatic embryogenesis and organogenesis in peanut: The role of 
thidiazuron and N-6-benzylaminopurine in the induction of plant 
morphogenesis. Plant Growth Regulation 28, 9-15.

Victor, J.M.R., Murthy, B.N.S., Murch, S.J., KrishnaRaj, S. and Saxena, 
P.K. (1999) Role of endogenous purine metabolism in thidiazuron-induced 
somatic embryogenesis of peanut (Arachis hypogaea L.).  Plant Growth 
Regulation 28, 41-47.

and in tomato

GILL, R., MALIK, K.A., SANAGO, M.H.M. and SAXENA, P.K. (1995) Somatic    
                embryogenesis and plant regeneration from seedling 
cultures of tomato (Lycopersicon esculentum Mill.). Journal of Plant 
Physiology 147, 273-276.

I used as well this protocol with very good results in tomato, pepper 
and aubergine.

Giorgis Kaparakis

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