Re: Xanthi RNA prep

Subject: Re: Xanthi RNA prep
From: Jeffrey Lerner <jeffreylerner@WORLDNET.ATT.NET>
Date: Tue, 6 Feb 2001 21:10:04 -0500
Organization: Dept.of Microbiology, University of Massachusetts
Reply-To: jeffreylerner@worldnet.att.net
Sender: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>

Sara Spencer wrote:
>
> Hello all!
> I am searching for a new way to prep RNA from transgenic tobacco tissue, for
> the TRizol I have been using isn't doing a great job.  The more simplified
> the better, seeing that i am screening through hundreds of plants!
>
> Thanks a lot,
> sara spencer

Dear Sarah,
  I used the RNAeasy plant mini-kit from Qiagen to build a cDNA library
from carrot roots. It worked very well for me. Would be glad to provide
more details, if necessary. Hope that helps.
Regards,
Jeff Lerner

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