Re: Contamination

Subject: Re: Contamination
From: "Schopke, Christian" <cschopke@VALIGEN.NET>
Date: Wed, 7 Feb 2001 11:54:13 -0500
Reply-To: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>
Sender: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>

Swati,

these refernces have been posted to the list last year, they all deal with
pink-pigmented methylotrophic bacteria. Apparently these bacteria can
persist for a long time in cultured tissues without being noticed because of
their specific growth requirements. PPFMs can be detected by culture on a
medium with methanol (see http://henson1.ssu.edu/~mahollan/leaf.html)

Prevention of pink-pigmented methylotrophic bacteria contamination of plant
tissue cultures" published in Plant Cell Reports (1996) 16:222-225, by
Sermsiri Chanprame

Holland, Mark A.; Polacco, Joseph C. (1994) PPFMs and other covert
contaminants: Is there more to plant physiology than just plant? Annual
Review of Plant Physiology and Plant Molecular Biology 197-209.

Holland, Mark A. (1997) Occam's razor applied to hormonology: Are cytokinins
produced by plants? Plant Physiology 115(3):865-868.

Good luck,

Christian

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