Re: Problem in selection, please suggest.

["Sage, Darren" <Darren.Sage@HRI.AC.UK>]

We regularly use 400 mg/l of cefotaxim with Narcissus and Prunus without any
problems.

Darren

-----Original Message-----
From: Ornela Wilck [mailto:wilck@LANDW.UNI-HALLE.DE]
Sent: 08 February 2001 12:07
To: PLANT-TC@TC.UMN.EDU
Subject: Re: Problem in selection, please suggest.

Hallo Chandra,
I'm not working on transformation etc. But the Cefotaxim concentration
is quite high (do You really need that much of it?) As to my knowledge
maximum concentrations of  250 mg/l are sufficient in plant tissue
cultures. Hope this helps.

Ornela

Boosani Chandra shekhar schrieb:

> Dear all,
> I am working on trasformation of indica rice using
> Agrobacterium.
> I am transfering the cocultivated callus to selection
> medium containing Cefotaxim at 500mg/L and Hygromycin
> at 50mg/L along with 3mg/L concentration of 2,4-D.
> All the time i could find the callus turning into pale
> brown and are not proliferation even after 6 weeks on
> selection.
> please suggest with some clues that may help me in
> proliferation the transformed callus on selection
> medium.
>
> Thanking you in advance
> Chandra Shekhar

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