Plant-tc Monthly Archive - February 2001

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Re: anthocyans in plant tissue cultures



Hello,
'I have never investigated this, but some time ago when I was working on
somatic embryogenesis in birch in connection with COST 87, we noticed that
the embryogenic cultures allways had red colour (anthocyanins, we
presumed), while the non-embryogenic of the same genotype never had any
anthocyanins present.  When preparing protoplats from these cultures, the
protoplats were also pretty pink from the embryogenic type callus.  I
assumed that the anthocyanins were in someway connected with the
regeneration ability and vaguely remember having seen some paper on this.
I would have liked to persue the idea, but the funding for birch ran out..
I don't know if this was much help since I do not really answer your
question, just some thoughts on the subject.
Trine

>Dear all,
>
>Could anyone explain me the phenomenon of anthocyans synthesis in the
>protoplast derived calli cultures. When and why anthocyans can be syntetised
>in callus or other in vitro plant cultures? Aditionally  I'm working on
>tomato and Solanum lycopersicoides protoplast cultures.
>Thank you for help!
>
>
>Mariola Kulawiec
>Warsaw Agricultural Univ,
>Dept. of Genetics'
>Warsaw, Poland
>markul@excite.com
>
Dr. Trine Hvoslef-Eide
Cell- and Tissue Culture Group
Agricultural University of Norway
Dept. of Horticulture & Crop Sciences
P.O.Box 5022, 1432 Aas, NORWAY
trine.hvoslef-eide@ipf.nlh.no
http://www.nlh.no/ipf/

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