Re: Benlate

[David Barrett <DBARRETT@EM.AGR.CA>]

I have used 25-50 mg/l autoclaved in the medium for in vitro germination 
with success.
Dave

>>> "Dr. Saifullah Khan" <drsaif@SUPER.NET.PK> 02/12 8:51 PM >>>
Thank you for mentioning the mistake
we generally add about 2-2.5mg/l (Not gram) in the media before autoclaving 
to control fungal contamination. For us it works effectively  it seems this 
is heat stable. For blanching we make 10mg/l solution to dip the roots of 
plants in lukewarm water just before transplanting in vitro material to 
green house

Best Regards to every one
Dr. Saifullah Khan Rao

David Sims wrote:

> Hello,
>
> Can Benomyl be heat sterilized? You mention a concentration of 2
> grams/liter. Is this correct? It seems like quite a large amount.
>
> Thank you,
> David Sims
>
> "Dr. Saifullah Khan" wrote:
> >
> > dear Ramkumar'
> >  benlate is a fungicide produced by Dupont company
> > Its chemical name is Benomyle (Methyl 1-(butylcarbamoyl)-2-
> > Benzizimidazolecarbamate     50% it contain 50 % inert material
> > this can be used 2 gram/liter generally to control fungus in tissue culture
> > plants during transfer and acclimatization.
> > Hope this will give the required information's
> > Dr. Saifullah Khan Rao
> > HEJ research Institute
> > University of Karachi Pakistan
> >
> > Ramkumar-S-Exe-R&D-Sug-EID wrote:
> >
> > > Dear All,
> > >      Can anyone in the list provide information's on Benlate - its chemical
> > > name, its availability, usage and concentration for controlling fungal
> > > contamination in Plant tissue culture media?
> > > Thanks in advance.
> > >
> > > With regards
> > > Ramkumar.S
> > > My internet id: ramkumar_in@yahoo.com
> > > *******Nothing dies faster than an idea in a closed mind*******

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