Re: Problem with 2,4-D--- solution

[sharon <sharon.williams@MAILBOX.UQ.EDU.AU>]

Dear Chandra,

to dissolve 2,4-D we do as follows.

dissolve measured powder in small volume of absolute Ethanol
add ddH20 up to required volume.
pH solution to between 6 and 7 with NaOH to prevent powder coming out of
solution.
(N.B. the solutionis very sensitive to pH changes... single drops of NaOH
can swing pH from 3 to 9...so be patient).

*******************************
Sharon Williams
B. App.Sc.(Biotech) Hons
Research Assistant
School of Land and Food Sciences
University of Queensland
St Lucia   QLD  4072
(W)  3365 1518
(H)   3371 4489
(F)    3365 1177
****************************
There are two rules for ultimate success in life.
1. Never divulge everything you know.


----- Original Message -----
From: "Boosani Chandra shekhar" <boosani2001@yahoo.com>
To: <plant-tc@tc.umn.edu>
Sent: Tuesday, February 20, 2001 4:20 PM
Subject: Problem with 2,4-D


> Dear All,
>
> I am preparing 1mM stock of 2,4-D by dissolving it in
> 5ml of Absolute Ethanol and then making up the volume
> to 50ml with sterile water. I even tried the same
> using 2mM NaOH. But in either case i could find
> precipitation of 2,4-D.
> The source of my Double distilled water is a Quartz
> distillation unit to which a resin based water
> softener is attached.
> Please suggest.
> Chandra Shekhar

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