Re: How to Perform a Partial Digestion!

[Mahmoud Yaish <degmya@UNILEON.ES>]

> Dear Friends,
>
> I am doing plasmid construction and will use partial digestion for it. But I
> don't know how long is good for partial digestion for a standard reaction. I
> really appreciate your suggestions.
>
> Yours sincerely,
>
> Daolin Fu
> Kansas State University
>
> Tel: 785 532 7226
> Email: dlfu@ksu.edu
>
Dear Daolin,
I think you can do partial digestion using at least two of the following methods:
Check the minimum time that is necessary to realize your fragment during the digestion or
using ethidium bromide at several concentrations in your digestion procedure.
Sincerely Yours,

Mahmoud

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