Re: Fungal infection?

[Tom Stubblefield <tdstubb@SOFNET.COM>]

Hello Steve,
NaDCC is sodium dichloroisocyanurate. It is sold under the names
Trichloroisocyanuric acid, Trichlor-s-triazinetrione, or Trichlor at such
places as WalMart, K-Mart, Home Depot, etc. as a swimming pool disinfectant.
For soaking plants or plant parts for 10-30 minutes, the recommended
solution is 1/4 teaspoon to 1 cup water. Longer soaking times - use 1/8 tsp.
to 5 cups water. As an addition to the tc medium, 1/8 teaspoon to 3 cups
water to make a stock solution, then add 20ml to tissue culture medium and
process. This information was taken from Carol Shift's website so I am
including the URL.
http://www.kitchenculturekit.com/nadcc.htm.
I wonder how this compares to PPM?
Hope this helps somewhat.
Have a good one!
Tom

Hope this has been of some help.
Tom

----- Original Message -----
From: "Stephen Leivers" <capeseed@IAFRICA.COM>
To: <plant-tc@tc.umn.edu>
Sent: Friday, January 04, 2002 12:23 AM
Subject: Re: Fungal infection?

> Hi Thomas
> I am interested in  NaDCC. What is it and what concentration do you use it
> at? Do you add it before autoclaving?? Steve
>
> > -----Original Message-----
> > From: Plant Tissue Culture [mailto:plant-tc@tc.umn.edu]On Behalf Of Tom
> > Stubblefield
> > Sent: 03 January 2002 11:10
> > To: plant-tc@tc.umn.edu
> > Subject: Re: Fungal infection?
> >
> >
> > Does Benalte work better than NaDCC? The latter is definitely
> > autoclavable.
> > Has anyone done any work in carnivorous plants using NaDCC? Any
> > ill effects?
> > Thank you.
> > Thomas Stubblefield
> >
> >
> > ----- Original Message -----
> > From: "Elaine Lu" <ElaineLu@TELEPORT.COM>
> > To: <plant-tc@tc.umn.edu>
> > Sent: Thursday, January 03, 2002 2:58 PM
> > Subject: Re: Fungal infection?
> >
> >
> > > Yes, we include Benlate in the medium before autoclaving.  I believe it's
> > > autoclavable.  It's hard to tell how efficient this product is, since I
> > > never did a comparison  test with the same stock plant.  I do think it helps
> > > a lot since the contamination in my initiation cultures are mostly bacteria.
> > > We move cultures from initiation medium to stage II multiplication medium in
> > > 2 to 3 weeks.  The inclusion of Benlate in the initiation medium doesn't
> > > seem to make a difference in plants' later development.
> > >
> > > Elaine Lu, Ph.D.
> > > Oregon Plant Tissue Culture Lab
> > >
> > >
> > > ----- Original Message -----
> > > From: JOSE M. COLMENERO <idi@VIVEROSSEVILLA.COM>
> > > To: <plant-tc@tc.umn.edu>
> > > Sent: Wednesday, January 02, 2002 11:46 PM
> > > Subject: Re: Fungal infection?
> > >
> > >
> > > > You mean you use benlate in the esterile agar medium?
> > > > How efficient is this product for fungal inhibition?
> > > > Does it affect plant growing at any degree?
> > > > Is benlate autoclavable?
> > > >
> > > > Thank you for the information
> > > >
> > > > Jose M. Colmenero
> > > > Biotechnology PhD
> > > > R+D Department, Viveros Sevilla, S.A.
> > > > Ctra. Sevilla-Tocina, Km 20
> > > > 41310-Brenes, Sevilla
> > > > Spain
> > > > Tph +34-955 655 920
> > > > Fax  +34-955 655 921
> > > > Email: idi@viverossevilla.com