Re: double transformation

[Kristin Summerfelt <kristin.summerfelt@LSBC.COM>]

Alejandra,

 There has been a fair amount of research on this subject.  I refer you to
this paper for a good start on the subject.  I have personal experience with
both methods.  Results vary with host species, Agrobacterium strain, gene of
interest, etc.

M. Daley, V.C. Knauf K.R. Summerfelt, J.C. Turner 1998. Co-transformation
with one Agrobacterium tumefaciens strain containing two binary plasmids as
a method for producing marker-free transgenic plants. Plant Cell Reports 17:
489-496

Kristin R. Summerfelt
Associate Scientist
Large Scale Biology Corp
3333 Vaca Valley Pkwy
Suite 1000
Vacaville, CA 95688
P 707- 469-2386
F 707- 446-8654
www.lsbc.com

-----Original Message-----
From: Plant Tissue Culture [mailto:plant-tc@tc.umn.edu]On Behalf Of
malvarez@ffyb.uba.ar
Sent: Monday, January 07, 2002 6:13 AM
To: plant-tc@tc.umn.edu
Subject: double transformation

Hi everybody, Happy new year!
Here, in Argentina we are still working. I will appreciate any
information about double transformation by using different strains of
Agrobacterium tumefaciens.Is there any established
technique?Has anyone  experience on that kind of transformation?
I hope that someone could give me his advice. Alejandra