Re: double transformation

[Kristin Summerfelt <kristin.summerfelt@LSBC.COM>]

Dear Aaron,

I wish I could answer your question but I have not paid attention to the
current status of the intellectual property pertaining to this subject.  I
suggest that you do a patent search.  Here is a good place to start,
http://www.uspto.gov/

Kristin

Kristin R. Summerfelt
Associate Scientist
Large Scale Biology Corp
3333 Vaca Valley Pkwy
Suite 1000
Vacaville, CA 95688
P 707- 469-2386
F 707- 446-8654
www.lsbc.com


-----Original Message-----
From: Plant Tissue Culture [mailto:plant-tc@tc.umn.edu]On Behalf Of Aaron
Zelcer
Sent: Tuesday, January 08, 2002 12:01 AM
To: plant-tc@tc.umn.edu
Subject: Re: double transformation

Kristin Summerfelt wrote:

>Subject: Re: double transformation
>
>Alejandra,
>
>  There has been a fair amount of research on this subject.  I refer you to
>this paper for a good start on the subject.  I have personal experience with
>both methods.  Results vary with host species, Agrobacterium strain, gene of
>interest, etc.
>
>M. Daley, V.C. Knauf K.R. Summerfelt, J.C. Turner 1998. Co-transformation
>with one Agrobacterium tumefaciens strain containing two binary plasmids as
>a method for producing marker-free transgenic plants. Plant Cell Reports 17:
>489-496
>
>Kristin R. Summerfelt


Dear Kristin,

I wonder what is the patent situation regarding the co-transformation
approach for generating marker-free transgenics, effected by 2 agro
strains, 1 strain containing 2 different binaries, or by a single
binary harboring multiple T-DNAs. Can you please share with us your
expertise on this issue?

Thanks,

Aaron Zelcer