Re: a question about vitrification

[Orlando Mejia <mejia_orlando@HOTMAIL.COM>]

Mr Kabiri:
I use 1.15 gr/lt of calcium nitrate Ca2(NO3)2 instead of amonium nitrate and
  I eliminated calcium cloride from MS medium on meristematic regeneration
of Dianthus cariophillus (carnations). It has reduced vitrification in the
culture in a very good percentage. I don't know if this can help you.
Best wishes,

Sandra Bonilla

>From: mohammad kabiri <kabirie2002@yahoo.com>
>Reply-To: Plant Tissue Culture <plant-tc@tc.umn.edu>
>To: plant-tc@tc.umn.edu
>Subject: a question about vitrification
>Date: Tue, 8 Jan 2002 05:56:15 -0800
>
>To whom it may concern
>Greetings. I face with vitrification in my
>regeneration of spinach. I've decided to decline
>NH4NO3 in medium. How much should I decline it ? and,
>which material is able to compensate the deficient of
>NH4NO3?
>Thank you in advance. i look forward to hear from you
>as soon as possible.
>
>Regards,
>M. Kabairi