Re: Time needed for vacuum infiltration

["Hüseyin Avni Öktem" <haoktem@METU.EDU.TR>]

Dear Rishi,

This depends very much on the tissue you are using. With lentil cotyledonary
node explants 200mmHg (among 400 and 600 mmHg) and 20 min ( vs 30 min)
resulted in best transient GUS expression in our experiments. However in
tobacco and potato leaves 5 min at 600 mmHg highly enhanced the expression
levels. Hope this will help..

Sincerely

Huseyin Avni OKTEM
Dept. of Biology
Middle East Technical University
Ankara-TURKEY
e-mail: haoktem@metu.edu.tr

-----Original Message-----
From: Plant Tissue Culture [mailto:plant-tc@TC.UMN.EDU]On Behalf Of
anantharama rishi
Sent: Friday, January 18, 2002 7:45 AM
To: plant-tc@TC.UMN.EDU
Subject: Time needed for vacuum infiltration

Dear All,
I would like to know that how much time is required to perform vacuum
infiltration for the agrobacterium to enter plant tissues like
germinating seeds/callus/leaves. What should be the vacuum conditions that
are needed? How much damage is done to the tissues with respect to
transcription and other cellular process. Looking forward for a good
discussion and thanks in advance
Rishi

Anantharama Rishi
University of Minnesota,
Duluth, Mn - 55812
E-mail: arishi@d.umn.edu