Re: Agrobacterium strains for monocot transformation

["Sage, Darren" <Darren.Sage@HRI.AC.UK>]

I suggest C58C1, it works with some bulbous monocots.

Darren

Dr Darren O. Sage
Horticulture Research International
Wellesbourne
Warwick
CV35 9EF

e-mail: Darren.Sage@hri.ac.uk
Tel: 01789 470382
Fax: 01789 470552

-----Original Message-----
From: Shathawy Matar [mailto:shathawy@ATTBI.COM]
Sent: 05 March 2002 21:47
To: PLANT-TC@TC.UMN.EDU
Subject: Agrobacterium strains for monocot transformation

Hi,
I am trying to transform embryogenic callus of a monocot
plant (phoenix dactylifera) and was wondeirng if any one
could recommend some agrobacterium strains that work
well with monocots and if there are any special
techniques (co-culure temp, sonification, etc.) that may
increase the frequency of transformation.
Thank you
 Shathawy
Colorado State university