PCR, southern blot and flanking region of T-DNA

[chen li <chen_li3@YAHOO.COM>]

Dear TC members:
      Among PCR, southern blot and analysis of
flanking region of T-DNA,which method is the best way
to demonstrate that T-DNA really integrates into plant
genome? What are the main advantages and disavantages
of these methods on demonstrating that T-DNA
integrates into plant genomes.

 Any idea and refernces will be highly appreciated.

Li