Plant-tc Monthly Archive - March, 2003

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Re: fungicide or surface sterilization protocol



Hello

Recently I used copper sulfate in a concentration of 50 mg/l (2000 fold
increase over that used in MS media)  in the media in order to inhibit
bacteria development. So far I tried it for only one cycle with nice result.
I used it for different Aracea ornamentals and  Paulonia. It seems that also
rooting is  nicely affected by increase copper level in the media. There
were no toxicity symptoms due to the elevated copper.

Shlomo

----- Original Message -----
From: "Eric Mercure" <EricM@PARAMOUNTFARMING.COM>
To: <PLANT-TC@LISTS.UMN.EDU>
Sent: Thursday, March 06, 2003 5:43 PM
Subject: Re: [PLANT-TC] fungicide or surface sterilization protocol
>
> Dear S. Sinha,
>
> The copper sulfate level is 200-fold increase over that used in MS media.
>
> --Eric
>
> -----Original Message-----
> From: S K Sinha [mailto:sinhask@TERI.RES.IN]
> Sent: Monday, March 03, 2003 8:26 PM
> To: PLANT-TC@LISTS.UMN.EDU
> Subject: Re: [PLANT-TC] fungicide or surface sterilization protocol
>
> Dear Eric
> Could you tell me what is the strength of copper sulfate. We also face the
> same problem in oranges
> Regards
> S K Sinha
> >
>> EricM@PARAMOUNTFARMING.COM 03/03/03 23:35 PM >>>
> Are the axillary buds breaking and/or actively growing?  It is easier to
> sterilize plant material at this stage.  Also, what contamination are you
> observing and how fast does it appear after initiating cultures?  If
> bacterial, PPM and/or additional copper sulfate works well for the trees I
> work with, along with transfers every 4 to 5 days for a few weeks.
>
> Regards,
> Eric Mercure
>
> -----Original Message-----
> From: Pijut, Paula [mailto:ppijut@FNR.PURDUE.EDU]
> Sent: Monday, March 03, 2003 9:44 AM
> To: PLANT-TC@LISTS.UMN.EDU
> Subject: [PLANT-TC] fungicide or surface sterilization protocol
>
>    Can anyone suggest a fungicide or surface sterilization protocol that
> works well for cleaning up field material (small branches with axillary buds
> from mature trees) prior to placing the nodal sections into culture?  We
> have tried several combinations of ethanol, bleach, and time in solutions,
> to no avail.  Any suggestions will be most appreciated.  Thanks.
>  --Paula


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