Plant-tc Monthly Archive - August, 2003

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Re: in vitro flowering

Hi Robert,

Re: in vitro flowering. We run into in vitro flowering pretty frequently,
usually with species that are very floriferous by nature, and I would say
that phlox falls into that category as well. Some things that may play a
role in precocious flowering in vitro: age of tissue used to begin with
(it's extremely difficult to reverse mature tissue's morphology into
something more juvenile).... (kind of like humans),  try reducing the amount
of sucrose and/or phosphorous in vitro, as well as the level of plant growth
regulators. For us, sucrose usually seems to have the greater effect, but it
definitely varies from one species to another!!   As one can see by the
comments in this discussion, there are many aspects of flowering that
science can't explain yet. If we can unlock the secrets of the "flowering
gene" with more species, we'll really be onto something!!! Good luck, and
keep us updated with what you find.

M.M. From
Lab for Rare & Endangered Plants
Omaha HDZoo

----- Original Message -----
From: "Kane, Michael E." <MKane@MAIL.IFAS.UFL.EDU>
Sent: Friday, August 15, 2003 12:40 PM
Subject: Re: [PLANT-TC] in vitro flowering

We have found that the addition of gibberellic acid will induce in vitro
flowering in various wetland/aquatic plant genera. Are using GA in the
medium?  Perhaps a low level of an GA antagonist/inhibitor may work to block
flowering.  However, it will also probably reduce growth.  In some species
flowering can be induced by ethylene.  Venting may alleviate this.

Mike Kane

Dr. Michael E. Kane, Professor
Environmental Horticulture Department
University of Florida
P.O. Box 110675
Gainesville, FL 32611
Office: 352-392-1831 ext. 205
Cell: 352-222-7724
Fax: 352-392-1413

-----Original Message-----
From: Dr. Joseph Arditti [mailto:jarditti@UCI.EDU]
Sent: Friday, August 15, 2003 1:26 PM
Subject: Re: [PLANT-TC] in vitro flowering

At 03:09 PM 15-08-03 +0500, you wrote:
>Dear J.Ardittti
>I have seen this phenomenon in my lab. and in one of our growth room
>accidently photoperiod was resvered. I mean 8 hours light and 16 hours
>dark when we corrected the mistak many dendrobium shows tiny bunches of
>flowers. I think the light and/or dark period have strong influence on
>invitro flowering

This is very possible and well worth pursuing because the subject has not
been studied intensively.


>Dr.Saifullah Khan Rao
>HEJ Karachi University
>----- Original Message -----
>From: "Dr. Joseph Arditti" <jarditti@UCI.EDU>
>Sent: Friday, August 15, 2003 11:04 AM
>Subject: Re: [PLANT-TC] in vitro flowering
> > At 07:44 AM 15-08-03 +0200, you wrote:
> > >Dear TCers
> > >
> > >the tiny shoots keep on flowering
> > >My question to the forum is: Are there any substances that can
> > >trigger back vegetative growth (cytokinins?), did anybody else see
> > >this phenomena
> >
> > I have not seen this exact phenomenon, but know that in orchids BA enhances
> > flowering in vitro. Could it be doing the same to Phlox?
> >
> > >Any help and ideas are welcome
> >
> > I have no idea what to do. Try an inhibitor like ABA?  Just guessing.
> >
> > Joseph Arditti

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