Re: Cell suspension

[Archana Gawde <sendtoarch@YAHOO.COM>]

Dear Elham

You need to initiate callus on a solid medium first and then u can go for 
suspension cultures
for callus induction you can try to culture the explant on MS (full strength 
or half strength) with range of 2,4 D or with NAA -BA combinations.

Archana

Elham Attaran <frau1976@YAHOO.COM> wrote:
Dear all,
I am working on establishing cell suspension culture for cactaceae family 
plant to induce secondary metabolites. Can anyone advise me what is a 
suitable medium (hormone, elicitor etc) to use for cactaceae?

Thank you in advance.