Re: sterilization of explant segments

[simona vesa <simona_vesa@YAHOO.COM>]

In my lab we are using DOMESTOS (you can find it in
supermarkets, is a cleaning product).
Steps:
- prepare a 10% solution (in dist. water)
- keep the bulbs in 70% alcohol for 1-2 minute(s) (in
a recipient with lid)
- remove the alcohol and replace with Domestos
- from there work in the hood
- keep the bulbs in Domestos for 20 to 30 minutes
- remove the alcohol and rince thoroughly (till there
is no foam left) with distilated sterile water

I hope that this will help.

Good luck,
Simona

--- Hamad Abu Zahra <hamad_genetics@YAHOO.COM> wrote:
> Dear all,
>
> i am wondering about chloramine(s) T, is it really an
> important step to sterilize the final segments of
> explants to be cultured?
>
> i've read that 075% conc. is useful for bulb
> sterilization ? is this right?
>
> is there any alternatives for it and how can i prepare
> it in the lab if not found?
>
> thanks all
> best regards
>
> Hamad Abu Zahra
> P.O.Box   2002   Um Alsummaq & Khalda
> Postal Code 11821
> Amman  -  Jordan
> Telephone :+962 6 582  78 88
> Mobile      :+962 777 65 65 20

PhD Student Simona Vesa
Babes-Bolyai University, Department of Experimental Botany
Plant Genetic Manipulation Group
5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania