Re: sterilization of explant segments

[simona vesa <simona_vesa@YAHOO.COM>]

The Domestos that we are using is a Unilever brand and
has 5% sodium hipoclorite.

--- "Dr. Joseph Arditti" <jarditti@UCI.EDU> wrote:

> At 02:46 AM 04-10-05, you wrote:
> >In my lab we are using DOMESTOS
>
>
> If I am not mistaken Domestos is a brand of Cloroz.
> What is the sodium
> hypochlorite concentration in it?
>
> Joseph Arditti
>
> >(you can find it in
> >supermarkets, is a cleaning product).
> >Steps:
> >- prepare a 10% solution (in dist. water)
> >- keep the bulbs in 70% alcohol for 1-2 minute(s) (in a recipient with lid)
> >- remove the alcohol and replace with Domestos
> >- from there work in the hood
> >- keep the bulbs in Domestos for 20 to 30 minutes
> >- remove the alcohol and rince thoroughly (till there
> >is no foam left) with distilated sterile water
> >
> >I hope that this will help.
> >
> >Good luck,
> >Simona
> >
> >--- Hamad Abu Zahra <hamad_genetics@YAHOO.COM> wrote:
> >
> > > Dear all,
> > >
> > > i am wondering about chloramine(s) T, is it really an
> > > important step to sterilize the final segments of
> > > explants to be cultured?
> > >
> > > i've read that 075% conc. is useful for bulb
> > > sterilization ? is this right?
> > >
> > > is there any alternatives for it and how can i prepare
> > > it in the lab if not found?
> > >
> > > thanks all
> > > best regards
> > >
> > > Hamad Abu Zahra
> > > P.O.Box   2002   Um Alsummaq & Khalda
> > > Postal Code 11821
> > > Amman  -  Jordan
> > > Telephone :+962 6 582  78 88
> > > Mobile      :+962 777 65 65 20
> >
> >
> Dr. Joseph Arditti, Professor Emeritus
> University of California, Irvine, CA 92604
> E-mail: jarditti@uci.edu
>

PhD Student Simona Vesa
Babes-Bolyai University, Department of Experimental Botany
Plant Genetic Manipulation Group
5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania