Re: Determination of Developmental stage of ovules

["Reinerio RB. Benega" <rbenega@BIOPLANTAS.CU>]

Dear Delphine,
It is not easy to determine the developmental stage for isolated ovule.
Normally, researcher are going to determine the developmental stage in
anther tissue, microspore and to relate one to another. That is especially
for isolated ovule culture in a breeding program/tissue culture for plant
mass propagation.

I hope it can help you in some way.

Good luck,

Reinerio.

Dr. Reinerio Benega García.
Director de Producción.
Centro de Bioplantas. Universidad de Ciego de Avila (UNICA)
Carretera a Morón. Km 9. C.P.: 69 450. Cuba.

Tel.:(53 33) 22 5768/22 4016
FAX:(53 33) 26 6340/26 6365
E-mail: reinerio_benega@yahoo.com
http://www.bioplantas.cu/

-----Original Message-----
From: Delphine Amah <dellysparkle@YAHOO.COM>
To: PLANT-TC@lists.umn.edu
Date: Thu, 6 Oct 2005 04:18:08 -0700
Subject:  Determination of Developmental stage of ovules

> Dear Tcers,
>
> I need help with a staining/light microscopy or other
> simple technique that can be used to determine the
> developmental stages of ovules(un-pollinated) and
> their viability.
>
> Any clues??
>
> Kind regards,
>
>
> Amah Delphine
> Breeding program
> International Institute of Tropical Agriculture,
> B.P.2008, Messa-Yaounde,
> Cameroon.
> Office Tel: (237) 223 75 22 Ext 102/121  mobile: (237) 7768275
>
> "I have not failed, I have only found ten thousand ways that wont work!"