Re: Determination of Developmental stage of ovules

[Delphine Amah <dellysparkle@YAHOO.COM>]

Many thanks Dr Reinerio!
Trouble is here I am looking at the genus Musa where
female flowers are different from male flowers; the
female coming out before the male.

Rgds,
Delphine

--- "Reinerio RB. Benega" <rbenega@BIOPLANTAS.CU> wrote:

> Dear Delphine,
> It is not easy to determine the developmental stage
> for isolated ovule.
> Normally, researcher are going to determine the
> developmental stage in
> anther tissue, microspore and to relate one to
> another. That is especially
> for isolated ovule culture in a breeding
> program/tissue culture for plant
> mass propagation.
>
> I hope it can help you in some way.
>
> Good luck,
>
> Reinerio.
>
>
> Dr. Reinerio Benega García.
> Director de Producción.
> Centro de Bioplantas. Universidad de Ciego de Avila
> (UNICA)
> Carretera a Morón. Km 9. C.P.: 69 450. Cuba.
>
> Tel.:(53 33) 22 5768/22 4016
> FAX:(53 33) 26 6340/26 6365
> E-mail: reinerio_benega@yahoo.com
> http://www.bioplantas.cu/
>
>
> -----Original Message-----
> From: Delphine Amah <dellysparkle@YAHOO.COM>
> To: PLANT-TC@lists.umn.edu
> Date: Thu, 6 Oct 2005 04:18:08 -0700
> Subject:  Determination of Developmental
> stage of ovules
>
> > Dear Tcers,
> >
> > I need help with a staining/light microscopy or other
> > simple technique that can be used to determine the
> > developmental stages of ovules(un-pollinated) and
> > their viability.
> >
> > Any clues??
> >
> > Kind regards,

Amah Delphine
Breeding program
International Institute of Tropical Agriculture,
B.P.2008, Messa-Yaounde,
Cameroon.
Office Tel: (237) 223 75 22 Ext 102/121  mobile: (237) 7768275

"I have not failed, I have only found ten thousand ways that wont work !"