Re: Determination of Developmental stage of ovules

["Dr. Lorenzo Burgos" <burgos@CEBAS.CSIC.ES>]

Dear Delphine,
we have used often a staining procedure to determine the development stage
of apricot ovules and similar procedures are used with other fruit trees.
It consists of inclusion in parafin, serial sectioning and staining with
different dyes such as safranin.
However, I would not called it a simple technique. If you only are
interested in ovule viability I remember of some papers where a
fluorocromatic staining is used in a much simpler way, but they first made a
comparison between both methods in order to be sure of what their results
meant.
If you are still interested, please contact me in my personal address
instead of the general one.
___________________________________________________________
Dr. Lorenzo Burgos
Investigador Científico
Departamento de Mejora de Plantas
CEBAS-CSIC
Aptd. 164, 30100 Murcia (España)
Telf. +34 968 396317/6200
Fax  +34 968 396213

----- Original Message -----
From: "Delphine Amah" <dellysparkle@YAHOO.COM>
To: <PLANT-TC@LISTS.UMN.EDU>
Sent: Thursday, October 06, 2005 1:18 PM
Subject:  Determination of Developmental stage of ovules

> Dear Tcers,
>
> I need help with a staining/light microscopy or other
> simple technique that can be used to determine the
> developmental stages of ovules(un-pollinated) and
> their viability.
>
> Any clues??
>
> Kind regards,
>
> Amah Delphine
> Breeding program
> International Institute of Tropical Agriculture,
> B.P.2008, Messa-Yaounde,
> Cameroon.
> Office Tel: (237) 223 75 22 Ext 102/121  mobile: (237) 7768275
>
> "I have not failed, I have only found ten thousand ways that wont work !"