Flow cytometer

[Y Shi <shiyz06591@HOTMAIL.COM>]

Hi all

I want to check the chromosome number variation of canola cell suspensions.
DI Josef Schmidt suggested flow cytometer. I am going to use it. Thank you
very much, DI Josef Schmidt!

However, I do not have experience in using flow cytometry to determine cell
ploidy level. Does any one have a protocol? Any experience is very welcome.

Particularly: 1) Should I use diploid canola as standard to compare with our
suspension cells? How to normalize all the cells to G1 phase?

Thank you very much!

YZ