Re: Flow cytometer

[Schmidt Josef <josef.schmidt@ARCS.AC.AT>]

Dear Y Shi,
good to hear you are going for flow cytometry.
Here is a link to one of our partner institutes with more recent expertise
in this area.

http://www.ueb.cas.cz/Olomouc1/LabDol/Research/Flow_cytometry/labdol_flowcytometry.htm

If you send your email address to josef.schmidt@arcs.ac.at, I can also
send you a copy of a paper from our department.
All the best
Josef

-----Ursprüngliche Nachricht-----
Von: Plant Tissue Culture im Auftrag von Y Shi
Gesendet: Do 06.10.2005 18:37
An: PLANT-TC@LISTS.UMN.EDU
Betreff:  Flow cytometer

Hi all

I want to check the chromosome number variation of canola cell suspensions.
DI Josef Schmidt suggested flow cytometer. I am going to use it. Thank you
very much, DI Josef Schmidt!

However, I do not have experience in using flow cytometry to determine cell
ploidy level. Does any one have a protocol? Any experience is very welcome.

Particularly: 1) Should I use diploid canola as standard to compare with our
suspension cells? How to normalize all the cells to G1 phase?

Thank you very much!

YZ