Plant-tc Monthly Archive - October, 2005

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Re: alcohol in autoclave?



At 09:18 AM 14-10-05, you wrote:
Hi Elena,

It's not good practice though for students to get used to this.  You could
put up a sign beside the autoclave that says "Do not autoclave
ethanol."   And you could also warn your students at least 3 times during
a class that if any group does this, you're going to dock 5 marks per
student in the group.  That will get their attention.

Docking always works. I seldom had the heart to do it.

Joseph Arditti


Lizette


"Dr. Elena Yasnetskaya" <housewife@mail.ru> wrote: From: Dr. Elena Yasnetskaya <housewife@mail.ru> Subject: Re[2]: alcohol in autoclave? Date: Fri, 14 Oct 2005 10:51:22 +0400

Dear Lizette,
Thank you for your reply; I am worried about ethanol behaviour in
autoclave because my students use to place it for sterilization, together
with all used tools and jars, after transfers. We sterilize instruments in
70% ethanol, then in flame. Then everything have to be sterilized in
autoclave, except (I told them few times!) the glass with ethanol. But
they were forgetting to throw ethanol in to the sink. When other
colleagues see this ethanol nearly in autoclave, they call my students a
'bomb makers".
Any how, now I am sure that there is no risk of explosion.
Thanks again,
Elena

With the highest regards and appreciation
Dr. Elena Yasnetskaya
Department of Plant Health
Plant Biotechnology Centre
Annibale Preca Street
Lija BZN 04, MALTA

Tel/Fax: + 356 21 374610

Dr. Joseph Arditti, Professor Emeritus University of California, Irvine, CA 92604 E-mail: jarditti@uci.edu


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