Re: tube capping

[Hamad Abu Zahra <hamad_genetics@YAHOO.COM>]

Dear all,

many thanks for your answers, but can we consider para
film dangerous for increasing the risk of
contamination? i dont think breathing is a problem for
cultures as they are subcultured/transfered every now
and then.

thank you all again.

best regards

--- "Carol M. Stiff" <kck@TURBONET.COM> wrote:

> Para film keeps out dust and "critters" and does have some breathing
> capabilities though I'm not sure if has been documented and will differ with
> the number of layers of Para film you use.  People also use surgical tape,
> saran wrap, Nescofilm, micropore tape, florists tape, Austraseal, etc.
> Breath ability differs on all of these and documentation is limited.
> Austraseal is advertised as having better breathable characteristics but
> again documentation is limited.  Make a nice master's student project???
>
> carol
>
> Carol M. Stiff, Ph.D.
> Kitchen Culture Kits, Inc.
> http://www.kitchenculturekit.com
> 936-699-3551   FAX 936-699-3553
> kck@turbonet.com
> For translation, go to:
> http://babelfish.altavista.com/
>
> -----Original Message-----
> From: Plant Tissue Culture
> [mailto:PLANT-TC@LISTS.UMN.EDU] On Behalf Of Hamad Abu Zahra
> Sent: Sunday, October 16, 2005 5:33 AM
> To: PLANT-TC@LISTS.UMN.EDU
> Subject:  tube capping
>
> greetings all,
>
> i wonder why we use a parafilm after capping test tubes filled with media
> and cultured explants?
>
> is parafilm even sterile? does it allow gas exchange?
>
> thanks
>
> best regards
>
> Hamad Abu Zahra
> P.O.Box   2002   Um Alsummaq & Khalda
> Postal Code 11821
> Amman  -  Jordan
> Telephone :+962 6 582  78 88
> Mobile      :+962 777 65 65 20

Hamad Abu Zahra
P.O.Box   2002   Um Alsummaq & Khalda
Postal Code 11821
Amman  -  Jordan
Telephone :+962 6 582  78 88
Mobile      :+962 777 65 65 20