Re: PLANT TISSUE PROBLEMS

["Mercure, Eric" <EricM@PARAMOUNTFARMING.COM>]

I have never seen micronutrients used at such high levels.  Are you
correct in saying that you use 11,065 mg per liter of manganese sulfate?
Is this common for other types of specialty medias that members of the
listserve use?

Regards,
Eric Mercure
Propagation Lab Manager
Paramount Farming Company
Bakersfield, CA

-----Original Message-----
From: Plant Tissue Culture [mailto:PLANT-TC@LISTS.UMN.EDU] On Behalf Of H40
Sent: Tuesday, October 18, 2005 10:12 AM
To: PLANT-TC@LISTS.UMN.EDU
Subject:  PLANT TISSUE PROBLEMS

Hi,

We are working on cucumber and melon haploidies, based on pollen
irradiation technique.

We are using E20A as nutrient media.

E20A

Macros
                                               mg/lt
KNO3                                    1075
NH4NO3                                 619
MgSO4.7H20                          206
CaCl2.2H20                            157
KH2PO4                                  71
Ca(NO3)2.H20                          25
NaH2PO4.4H20                        19
(NH4)2 SO4                              17
KCl                                           3,5


Micros

MnSO4.7H2O                        11065
ZnSO4.7H2O                          1812
H3BO3                                  1575
KI                                           345
Na2MoO42H2O                          94
CuSO4 5H2O                               8
CoCl2.6H2O                                8


FeChelat

Na2EDTA                                  37,30
FeSO4.7H20                               27,80



Meso Inosital                               50,30

Prydoxine   HCl                             5,50

Nicotinic Acid                                0,70

Thiamine HCl                                0,60

Calcium Pentotenat                          0,50

Biotine                                          0,005

Glycine                                          0,100

IAA                                               0,01

Sakkaroz                                         20 gr/lt

Agar                                                8 gr/lt (now 10
gr/lt)

pH                                                  5,9

autoclav                                  121 C----15 Minutes




We have a problem in melon plantlets. As you can see in attached
pictures the growth of melons is very very slow comparing to cucumber
plantlets.

our problem is in melons, and this problem is so slow growth (mostly
stopping); I mean
that the plants can not give new leaves, there is a stress but we dont
understand the reason of this. Same stress is valid in rooting of
plantlets.

Is there anybody have idea and experience regarding this subject?
Thanks in advance.

PS; I learned this group from a member of this group.