Re: Fw: PLANT TISSUE PROBLEMS

["Dr. RONALD de FOSSARD" <rfossard@AUSTARNET.COM.AU>]

Your medium looks to be very mild.  It's about half-strength minerals -
you could try doubling all concentrations.  Your growth regulators are
so low that I doubt if they are at all active - try doubling them.  You
are using only 0.1uM IAA.  Have you tried  higher concentrations? other
auxins?  You do not have any cytokinins.  These are the obvious things
to try first.  Otherwise we have to look to adding gibberellin to give
cultures a kick-along.  And occasionally sucrose (try lower
concentrations) and iron (try lower concentrations) can be inhibiting
growth.

The overall message is that you have a 'limiting' factor to be
discovered.  This could be some component of the medium or in the
incubation room.

Good Luck

----- Original Message -----
From: "H40" <haldunc@TNN.NET>
To: <PLANT-TC@LISTS.UMN.EDU>
Sent: Wednesday, October 19, 2005 3:56 AM
Subject:  Fw:  PLANT TISSUE PROBLEMS
>
> > Sorry, this is my fault.
> > I have corrected now. Sorry for this mistyping.
> >
> > ----- Original Message -----
> > From: "Mercure, Eric" <EricM@PARAMOUNTFARMING.COM>
> > To: <PLANT-TC@LISTS.UMN.EDU>
> > Sent: Tuesday, October 18, 2005 8:28 PM
> > Subject: Re:  PLANT TISSUE PROBLEMS
> >
> >
> > I have never seen micronutrients used at such high levels.  Are you
> > correct in saying that you use 11,065 mg per liter of manganese sulfate?
> > Is this common for other types of specialty medias that members of the
> > listserve use?
> >
> > Regards,
> > Eric Mercure
> > Propagation Lab Manager
> > Paramount Farming Company
> > Bakersfield, CA
> >
> > -----Original Message-----
> > From: Plant Tissue Culture [mailto:PLANT-TC@LISTS.UMN.EDU] On Behalf Of H40
> > Sent: Tuesday, October 18, 2005 10:12 AM
> > To: PLANT-TC@LISTS.UMN.EDU
> > Subject:  PLANT TISSUE PROBLEMS
> >
> > Hi,
> >
> > We are working on cucumber and melon haploidies, based on pollen
> > irradiation technique.
> >
> > We are using E20A as nutrient media.
> >
> > E20A
> >
> > Macros
> >                                               mg/lt
> > KNO3                                    1075
> > NH4NO3                                 619
> > MgSO4.7H20                          206
> > CaCl2.2H20                            157
> > KH2PO4                                  71
> > Ca(NO3)2.H20                          25
> > NaH2PO4.4H20                        19
> > (NH4)2 SO4                              17
> > KCl                                           3,5
> >
> > Micros
> >
> > MnSO4.7H2O                        11,065
> > ZnSO4.7H2O                          1,812
> > H3BO3                                  1,575
> > KI                                           0,345
> > Na2MoO42H2O                     0,094
> > CuSO4 5H2O                          0,008
> > CoCl2.6H2O                            0,008
> >
> > FeChelat
> > Na2EDTA                                  37,30
> > FeSO4.7H20                               27,80
> >
> > Meso Inosital                               50,30
> >
> > Prydoxine   HCl                             5,50
> >
> > Nicotinic Acid                                0,70
> >
> > Thiamine HCl                                0,60
> >
> > Calcium Pentotenat                          0,50
> >
> > Biotine                                          0,005
> >
> > Glycine                                          0,100
> >
> >
> > IAA                                               0,01
> >
> > Sakkaroz                                         20 gr/lt
> >
> > Agar                                                8 gr/lt (now 10 gr/lt)
> >
> > pH                                                  5,9
> >
> > autoclav                                  121 C----15 Minutes
> >
> >
> > We have a problem in melon plantlets. As you can see in attached
> > pictures the growth of melons is very very slow comparing to cucumber
> > plantlets.
> >
> > our problem is in melons, and this problem is so slow growth (mostly
> > stopping); I mean
> > that the plants can not give new leaves, there is a stress but we dont
> > understand the reason of this. Same stress is valid in rooting of
> > plantlets.
> >
> >
> > Is there anybody have idea and experience regarding this subject?
> > Thanks in advance.
> >
> > PS; I learned this group from a member of this group.