Re: about tween 20 on genetic transformation

[Dr Khalid M Khawar <kmkbhatti@YAHOO.COM>]

Dear XıBin
It was interseting to me to know that Tween 20 works. I will like reference to 
the paper.You can filter sterilise it (tween 20)  for your use.

xu bin <xubin3021@HOTMAIL.COM> wrote:
Dear all TCers,

I read some paper mentioned that mixing tween 20 with Agrobacterium can
enhance transformation rate using floral dip method. I wonder, how to
sterilize tween 20 (filter sterilization or autoclave) if I want to add
some into agrobacterium solution before co-culture. Thanks!

Best,

Bin Xu

With the highest regards and appreciation
Associate Prof. Dr. Khalid M Khawar
Tarimsal Biyoteknoloji
Tarla Bitkileri Birimi
Tarla Bitkileri Bol.
Ziraat Fakultesi
Ankara Univ. 06110,
Diskapi, Ankara, Turkiye